Group Blaas	Human rhinovirus-cell interaction and structural virology
			Group Clausen
Structural studies of complex protease machines
	Group Djinovic-Carugo	Structural studies of multi-protein interactions in muscle Z-disk
			Group Konrat
NMR spectroscopy and computational chemical biology
	Group Marlovits	Molecular machines: structure and function by cryo-EM
			Group Peters
Substrate recognition by the anaphase promoting complex/cyclosome
	Group Stolt-Bergner	Molecular Mechanisms of Membrane Transport and Membrane Protein Structural Biology
			Group Skern
Structure of viral proteins and their interaction partners

• DieterBLAAS
• Tim CLAUSEN
• KristinaDJINOVIC-CARUGO
• Robert KONRAT
• Tom MARLOVITS
• Jan-Michael PETERS
• Tim SKERN
• Peggy STOLT-BERGNER
  
  
   

Dieter Blaas

Publications:

Antibodies induced with recombinant VP1 from human rhinovirus exhibit cross-neutralization.
Edlmayr, J., K. Niespodziana, T. Popow-Kraupp, V. Krzyzanek, M. Focke-Tejkl, D. Blaas, M. Grote, and R. Valenta.
(2010).  Eur Respir J. (in press).

Liposomal leakage induced by virus-derived peptides, viral proteins and entire virions: rapid analysis by chip electrophoresis.
Weiss, V. U., Bilek, G., Pickl-Herk, A., Subirats, X., Niespodziana, K., Valenta, R., Blaas, D., and Kenndler, E.
(2010). Anal. Chem. (in press).

Uncoating of human rhinoviruses.
Fuchs R, Blaas D.
Rev Med Virol. 2010 May 7.

Atomic force microscopy studies of human rhinovirus topology and molecular forces.
Kienberger F, Zhu R, Rankl C, Gruber HJ, Blaas D, Hinterdorfer P.
Methods Enzymol. 2010;475:515-39.


In situ assembly of macromolecular complexes triggered by light.
Grunwald C, Schulze K, Reichel A, Weiss VU, Blaas D, Piehler J, Wiesmüller KH, Tampé R.
Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6146-51. Epub 2010 Mar 3.

Human Rhinovirus 14 Enters Rhabdomyosarcoma Cells Expressing ICAM-1 by a Clathrin, Caveolin, and Flotillin-independent Pathway.
Khan AG, Pickl-Herk A, Gajdzig L, Marlovits T, Fuchs R, Blaas D.
J Virol. 2010 Feb 3.

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Tim Clausen

Publications:

HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues.
Krojer T, Sawa J, Huber R, Clausen T.
Nat Struct Mol Biol. 2010 Jul;17(7):844-52. Epub 2010 Jun 27.

Molecular transformers in the cell: lessons learned from the DegP protease-chaperone.
Sawa J, Heuck A, Ehrmann M, Clausen T.
Curr Opin Struct Biol. 2010 Apr;20(2):253-8. Epub 2010 Feb 24.

Conversion of a regulatory into a degradative protease.
Hasenbein S, Meltzer M, Hauske P, Kaiser M, Huber R, Clausen T, Ehrmann M.
J Mol Biol. 2010 Apr 9;397(4):957-66. Epub 2010 Feb 22.

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Kristina Djinovic-Carugo

Publications:

Structural and functional characterisation of the chlorite dismutase from the nitrite-oxidizing bacterium "Candidatus Nitrospira defluvii": Identification of a catalytically important amino acid residue.
Kostan J, Sjöblom B, Maixner F, Mlynek G, Furtmüller PG, Obinger C, Wagner M, Daims H, Djinović-Carugo K.
J Struct Biol. 2010 Jun 22. [Epub ahead of print]PMID: 20600954 [PubMed - as supplied by publisher]Related citations

Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Mrs2, a magnesium ion transporter from yeast inner mitochondrial membrane.
Khan MB, Sjöblom B, Schweyen RJ, Djinović-Carugo K.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt 6):658-61. Epub 2010 May 26.

Opening of tandem calponin homology domains regulates their affinity for F-actin.
Galkin VE, Orlova A, Salmazo A, Djinovic-Carugo K, Egelman EH.
Nat Struct Mol Biol. 2010 May;17(5):614-6. Epub 2010 Apr 11.

Purification of recombinant growth hormone by clear native gels for conformational analyses: preservation of conformation and receptor binding.
Chen WQ, Salmazo A, Myllykoski M, Sjöblom B, Bidlingmaier M, Pollak A, Baumgärtel P, Djinovic-Carugo K, Kursula P, Lubec G.
Amino Acids. 2010 Aug;39(3):859-69. Epub 2010 Mar 19.

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Robert Konrat


Publications:

Identification of the gene encoding alkylglycerol monooxygenase defines a third class of tetrahydrobiopterin-dependent enzymes.
Watschinger K, Keller MA, Golderer G, Hermann M, Maglione M, Sarg B, Lindner HH, Hermetter A, Werner-Felmayer G, Konrat R, Hulo N, Werner ER.
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13672-7. Epub 2010 Jul 19.

The meandering of disordered proteins in conformational space.
Konrat R.
Structure. 2010 Mar 14;18(4):416

Pharmacophore Mapping via Cross-Relaxation during Adiabatic Fast Passage.
Auer R, Kloiber K, Vavrinska A, Geist L, Coudevylle N, Konrat R.
J Am Chem Soc. 2010 Feb 10;132(5):1480-1

Mutations in Caenorhabditis elegans him-19 Show Meiotic Defects that Worsen with Age.
Tang L, Machacek T, Mamnun YM, Penkner A, Gloggnitzer J, Wegrostek C, Konrat R, Jantsch M, Loidl J, Jantsch V.
Mol Biol Cell. 2010 Jan 13.

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Tom Marlovits

Publications:

Topology and organization of the Salmonella typhimurium type III secretion needle complex components.
Schraidt O, Lefebre MD, Brunner MJ, Schmied WH, Schmidt A, Radics J, Mechtler K, Galán JE, Marlovits TC.
PLoS Pathog. 2010 Apr 1;6(4):

Human Rhinovirus 14 Enters Rhabdomyosarcoma Cells Expressing ICAM-1 by a Clathrin, Caveolin, and Flotillin-independent Pathway.
Khan AG, Pickl-Herk A, Gajdzig L, Marlovits T, Fuchs R, Blaas D.
J Virol. 2010 Feb 3.

The Structure of the Salmonella typhimurium Type III Secretion System Needle Shows Divergence from the Flagellar System.
Galkin VE, Schmied WH, Schraidt O, Marlovits TC, Egelman EH.
J Mol Biol. 2010 Jan 11.

Type III secretion systems shape up as they ship out.
Marlovits TC, Stebbins CE.
Curr Opin Microbiol. 2010 Feb;13(1):47-52. Epub 2009 Dec 16. Review.




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Jan-Michael Peters

Publications:

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Neumann B, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, Ellenberg J.
Nature. 2010 Apr 1;464(7289):721-7

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM.
Science. 2010 Apr 30;328(5978):593-9. Epub 2010 Apr 1.

A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes.
Mazanek M, Roitinger E, Hudecz O, Hutchins JR, Hegemann B, Mitulović G, Taus T, Stingl C, Peters JM, Mechtler K.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Feb 15;878(5-6):515-24. Epub 2009 Dec 22.




Tim Skern

Acitivities:

Chair at the 6th YSA PhD Symposium, 16. - 17. June 2010, MUW

Publications:

Tim Skern´s book "Coffee House Notes on Virology" has been translated into Chinese.
ISBN 978-7-03-026886-0; order at:www.sciencep.com

• Dieter BLAAS
• Tim CLAUSEN
• Kristina DJINOVIC-CARUGO
• Robert KONRAT
  
• Tom MARLOVITS
  
• Jan-Michael PETERS
  
• Tim SKERN
• Peggy STOLT-BERGNER

Dieter Blaas

Publications:

Chip electrophoretic characterization of liposomes with biological lipid composition: Coming closer to a model for viral infection.
Bilek G, Weiss VU, Pickl-Herk A, Blaas D, Kenndler E.
Electrophoresis. 2009 Dec;30(24):4292-9.

Low pH-triggered beta-propeller switch of the low-density lipoprotein receptor assists rhinovirus infection.
Konecsni T, Berka U, Pickl-Herk A, Bilek G, Khan AG, Gajdzig L, Fuchs R, Blaas D.
J Virol. 2009 Nov;83(21):10922-30. Epub 2009 Aug 12.

Predictive bioinformatic identification of minor receptor group human rhinoviruses.
Weber C, Pickl-Herk A, Khan AG, Strauss S, Carugo O, Blaas D.
FEBS Lett. 2009 Aug 6;583(15):2547-51. Epub 2009 Jul 16.

Mimicking virus attachment to host cells employing liposomes: analysis by chip electrophoresis.
Weiss VU, Bilek G, Pickl-Herk A, Blaas D, Kenndler E.
Electrophoresis. 2009 Jun;30(12):2123-8.

Sensing picornaviruses using molecular imprinting techniques on a quartz crystal microbalance.
Jenik M, Schirhagl R, Schirk C, Hayden O, Lieberzeit P, Blaas D, Paul G, Dickert FL.
Anal Chem. 2009 Jul 1;81(13):5320-6.

Green fluorescent protein - Tagged HCV non-enveloped capsid like particles: development of a new tool for tracking HCV core uptake.
Katsarou K, Serti E, Tsitoura P, Lavdas AA, Varaklioti A, Pickl-Herk AM, Blaas D, Oz-Arslan D, Zhu R, Hinterdorfer P, Mavromara P, Georgopoulou U.
Biochimie. 2009 Jul;91(7):903-15. Epub 2009 May 4.

Conformation of Receptor Adopted upon Interaction with Virus Revealed by Site-Specific Fluorescence Quenchers and FRET Analysis.
Wruss J, Pollheimer PD, Meindl I, Reichel A, Schulze K, Schöfberger W, Piehler J, Tampé R, Blaas D, Gruber HJ.
J Am Chem Soc. 2009 Mar 30.

Receptor Arrays for the Selective and Efficient Capturing of Viral Particles.
Pollheimer PD, Kastner M, Ebner A, Blaas D, Hinterdorfer P, Gruber HJ, Howorka S.
Bioconjug Chem. 2009 Feb 5.

Human rhinovirus type 2 uncoating at the plasma membrane is not affected by a pH gradient but is affected by the membrane potential.
Berka U, Khan A, Blaas D, Fuchs R.
J Virol. 2009 Apr;83(8):3778-87. Epub 2009 Feb 4.

Site of human rhinovirus RNA uncoating revealed by fluorescent in situ hybridization.
Brabec-Zaruba M, Pfanzagl B, Blaas D, Fuchs R.
J Virol. 2009 Apr;83(8):3770-7. Epub 2009 Jan 21.

Virus analysis using electromigration techniques.
Kremser L, Blaas D, Kenndler E.
Electrophoresis. 2009 Jan;30(1):133-40. Review.

Minor group human rhinovirus-receptor interactions: geometry of multimodular attachment and basis of recognition.
Querol-Audí J, Konecsni T, Pous J, Carugo O, Fita I, Verdaguer N, Blaas D.
FEBS Lett. 2009 Jan 5;583(1):235-40. Epub 2008 Dec 13.


Activities:
Organizer of the Karl Landsteiner Symposium "100 Years of Poliovirus: From Discovery to Eridication", Austrian Academy of Sciences, 20th Nov, 2009

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Tim Clausen

Publications:

Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli.
Meltzer M, Hasenbein S, Mamant N, Merdanovic M, Poepsel S, Hauske P, Kaiser M, Huber R, Krojer T, Clausen T, Ehrmann M.
Res Microbiol. 2009 Nov;160(9):660-6. Epub 2009 Aug 18. Review.

Peptidic small molecule activators of the stress sensor DegS.
Hauske P, Mamant N, Hasenbein S, Nickel S, Ottmann C, Clausen T, Ehrmann M, Kaiser M.
Mol Biosyst. 2009 Sep;5(9):980-5. Epub 2009 May 8.

Structural basis of substrate specificity of plant 12-oxophytodienoate reductases.
Breithaupt C, Kurzbauer R, Schaller F, Stintzi A, Schaller A, Huber R, Macheroux P, Clausen T.
J Mol Biol. 2009 Oct 9;392(5):1266-77. Epub 2009 Aug 4.

McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR.
Fuhrmann J, Schmidt A, Spiess S, Lehner A, Turgay K, Mechtler K, Charpentier E, Clausen T.
Science. 2009 Jun 5;324(5932):1323-7.

Selectivity profiling of DegP substrates and inhibitors.
Hauske P, Meltzer M, Ottmann C, Krojer T, Clausen T, Ehrmann M, Kaiser M.
Bioorg Med Chem. 2009 Apr 1;17(7):2920-4. Epub 2009 Feb 5.

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Kristina Djinovic-Carugo

Publications:
Structural study of X-ray induced activation of carbonic anhydrase.
Sjöblom B, Polentarutti M, Djinovic-Carugo K.
Proc Natl Acad Sci U S A. 2009 Jun 30;106(26):10609-13. Epub 2009 Jun 11.PMID: 19520834

Can soaked-in scavengers protect metalloprotein active sites from reduction during data collection?
Macedo S, Pechlaner M, Schmid W, Weik M, Sato K, Dennison C, Djinović-Carugo K.
J Synchrotron Radiat. 2009 Mar;16(Pt 2):191-204. Epub 2009 Feb 25.PMID: 19240331

Structural basis of heme binding in the Cu,Zn superoxide dismutase from Haemophilus ducreyi.
Töro I, Petrutz C, Pacello F, D'Orazio M, Battistoni A, Djinović-Carugo K.
J Mol Biol. 2009 Feb 20;386(2):406-18. Epub 2008 Dec 11.PMID: 19103206

Terminal assembly of sarcomeric filaments by intermolecular beta-sheet formation.
Pinotsis N, Abrusci P, Djinović-Carugo K, Wilmanns M.
Trends Biochem Sci. 2009 Jan;34(1):33-9. Epub 2008 Nov 6. Review.PMID: 18996015

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Robert Konrat

Publications:
Measurement of signs of chemical shift differences between ground and excited protein states: a comparison between H(S/M)QC and R (1rho ) methods.
Auer R, Hansen DF, Neudecker P, Korzhnev DM, Muhandiram DR, Konrat R, Kay LE.
J Biomol NMR. 2009 Dec 22. [Epub ahead of print]PMID: 20033258

5-Fluoro pyrimidines: labels to probe DNA and RNA secondary structures by 1D 19F NMR spectroscopy.
Puffer B, Kreutz C, Rieder U, Ebert MO, Konrat R, Micura R.
Nucleic Acids Res. 2009 Dec;37(22):7728-40. Epub .PMID: 19843610

The protein meta-structure: a novel concept for chemical and molecular biology.
Konrat R.
Cell Mol Life Sci. 2009 Nov;66(22):3625-39. Epub 2009 Aug 19. Review.

Measuring the signs of 1H(alpha) chemical shift differences between ground and excited protein states by off-resonance spin-lock R(1rho) NMR spectroscopy.
Auer R, Neudecker P, Muhandiram DR, Lundström P, Hansen DF, Konrat R, Kay LE.
J Am Chem Soc. 2009 Aug 12;131(31):10832-3.

Autocorrelation analysis of NOESY data provides residue compactness for folded and unfolded proteins.
Schedlbauer A, Coudevylle N, Auer R, Kloiber K, Tollinger M, Konrat R.
J Am Chem Soc. 2009 May 6;131(17):6038-9.PMID: 19364097

Direct observation of the dynamic process underlying allosteric signal transmission.
Brüschweiler S, Schanda P, Kloiber K, Brutscher B, Kontaxis G, Konrat R, Tollinger M.
J Am Chem Soc. 2009 Mar 4;131(8):3063-8.

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Tom Marlovits

Activities:

Reviewer activity:

• Nature - Nature Microbilogy Reviews

• PLoS Pathogens

• Journal of Molecular Biology

 
Jan-Michael Peters

Publications:

Cohesin is required for higher-order chromatin conformation at the imprinted IGF2-H19 locus.
Nativio R, Wendt KS, Ito Y, Huddleston JE, Uribe-Lewis S, Woodfine K, Krueger C, Reik W, Peters JM, Murrell A.
PLoS Genet. 2009 Nov;5(11):e1000739. Epub 2009 Nov 26.PMID: 19956766

The cohesin complex is required for the DNA damage-induced G2/M checkpoint in mammalian cells.
Watrin E, Peters JM.
EMBO J. 2009 Sep 2;28(17):2625-35. Epub 2009 Jul 23.PMID: 19629043

Preventing carryover of peptides and proteins in nano LC-MS separations.
Mitulović G, Stingl C, Steinmacher I, Hudecz O, Hutchins JR, Peters JM, Mechtler K.
Anal Chem. 2009 Jul 15;81(14):5955-60.

HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.
Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L.
Curr Biol. 2009 May 26;19(10):816-26. Epub 2009 May 7.

Cohesin acetylation: from antiestablishment to establishment.
Peters JM, Bhaskara V.
Mol Cell. 2009 Apr 10;34(1):1-2.

How cohesin and CTCF cooperate in regulating gene expression.
Wendt KS, Peters JM.
Chromosome Res. 2009;17(2):201-14. Epub 2009 Mar 24. Review.

Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex.
Herzog F, Primorac I, Dube P, Lenart P, Sander B, Mechtler K, Stark H, Peters JM.
Science. 2009 Mar 13;323(5920):1477-81.PMID: 19286556

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Tim Skern

Publications:
Extension of flavivirus protein C differentially affects early RNA synthesis and growth in mammalian and arthropod host cells.
Schrauf S, Mandl CW, Bell-Sakyi L, Skern T.
J Virol. 2009 Nov;83(21):11201-10. Epub 2009 Aug 19.

Hydrolytic properties and substrate specificity of the foot-and-mouth disease leader protease.
Santos JA, Gouvea IE, Júdice WA, Izidoro MA, Alves FM, Melo RL, Juliano MA, Skern T, Juliano L.
Biochemistry. 2009 Aug 25;48(33):7948-58.

Aggregation-associated loss of antigenicity observed for denatured virion protein 1 of Equine rhinitis A virus in an enzyme-linked immunosorbent assay.
Kriegshäuser G, Kuechler E, Skern T.
Virus Res. 2009 Jul;143(1):130-3. Epub 2009 Mar 20.

Activities:
Organizer of the Karl Landsteiner Symposium "100 Years of Poliovirus: From Discovery to Eridication", Austrian Academy of Sciences, 20th Nov, 2009

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